Schematic representation of an AAV cis shuttle plasmid expressing
16. Februar 2017
eGFP under the CBA promoter and trans plasmid encoding for AAV2 2 rep proteins and capsid. Modified capsids used in this study contained four (AAV quadruple Y F) and six (AAV sextuple Y F) tyrosine to phenylalanine point mutations. (b) Experimental approach showing direct injection of AAV2 2 and simultaneous injection of red fluorescent microspheres into the CST.
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